Glutamate Receptor Subunit Membrane Expression AMPA receptor subunit membrane expression was evaluated by ï¬ow cytometry. Track cell division by FACS analysis of CFSE on FL-1 using a FACSCalibur. identify an endogenous immune surveillance mechanism to uncover a senolytic capacity of tissue-resident iNKT cells and pave the way for anti-senescence therapies that target these cells and their mechanism of activation. Analysis of flow cytometry data is performed by FlowJo 7.6 software. All values in the present study are presented as mean ± SD. Due to poor staining quality in Patient8, samples from this patient were excluded in downstream analysis of flow cytometry data. Data was analyzed using FlowJo software (Tree Star). Staining for E protein expression on the cell surface was comparable between the. was analyzed using FlowJo 10 (Becton Dickinson). Spleen tyrosine kinase (SYK) is a critical immune signaling molecule and therapeutic target. F G0 cell population of CSCs and parent cell lines determined by flow cytometry after Ki-67 and 7-AAD staining ( n = 3).
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